Electrochemically Reduced Water Protects Neural Cells from Oxidative Damage

Figure 4

Flow cytometric analysis of the intracellular H₂O₂ scavenging ability of ERW on N1E115 cells. N1E-115 cells (7.5 × 10⁴ cells) were treated with ERW, L-NAC (1.0 mM), AsA (1.0 mM), or control MQ-water, and incubated for 10 min ((a), (c), and (e)) or 24 h ((b), (d), and (f)). After each treatment, cells were harvested and resuspended in 1 ml PBS. Fluorescence intensities were measured immediately using an EPICS XL System II-JK flow cytometer with excitation and emission wavelengths of 495 and 525 nm, respectively. Histograms were analyzed by using FlowJo software provided with the cytometer.

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