Anandamide Protects HT22 Cells Exposed to Hydrogen Peroxide by Inhibiting CB1 Receptor-Mediated Type 2 NADPH Oxidase

Figure 6

AEA decreased intracellular ROS generation via CB1. The intracellular ROS levels were assessed by ROS reagent kit. (a)–(e) indicate the fluorescence intensity of ROS. (a) Control cells cultured in drug-free medium. (b) Cells exposed to 200 μM H₂O₂ for 3 h. (c) Cells exposed to 10 μM AEA plus 200 μM H₂O₂ for 3 h. (d) Cells exposed to 10 μM AEA plus 10 μM AM251 in the presence of 200 μM H₂O₂ for 3 h. (e) Cells exposed to 10 μM CB1 antagonist AM251 plus 200 μM H₂O₂ for 3 h. (f) Statistical results of (a)–(e). Results are expressed as means ± SD (𝑛=6). ^∗𝑃<0.05 versus the control (no H₂O₂, no AEA, and no AM251), ^#𝑃<0.05 versus the cells exposed to H₂O₂ alone, and ^∧𝑃<0.05 versus the cells exposed to AEA plus H₂O₂.

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