Anandamide Protects HT22 Cells Exposed to Hydrogen Peroxide by Inhibiting CB1 Receptor-Mediated Type 2 NADPH Oxidase
Figure 7
AEA increased intracellular SOD and ameliorated GSH/GSSG ratio. The cells were divided into six groups, Control: cells cultured in drug-free medium; AEA: cells exposed to 10 μM AEA for 3 h; H2O2: cells exposed to 200 μM H2O2 for 3 h; AEA + H2O2: cells exposed to 10 μM AEA plus 200 μM H2O2 for 3 h; AM251 + AEA + H2O2: cells exposed to 10 μM AEA plus 10 μM CB1 antagonist AM251 in the presence of 200 μM H2O2 for 3 h; AM251 + H2O2: cells exposed to 10 μM AM251 plus 200 μM H2O2 for 3 h. The intracellular SOD, GSH, and GSSG levels were assessed by the corresponding reagent kit, and the GSH/GSSG ratio was calculated according to the GSH and GSSG levels. (a) Intracellular SOD level. (b) Intracellular GSH level. (c) Intracellular GSSG level. (d) Intracellular GSH/GSSG ratio. Results are expressed as means ± SD (). versus control (no H2O2, no AEA, and no AM251), versus the cells exposed to H2O2 alone, and versus the cells exposed to AEA plus H2O2.
