Piperlongumine Induces Apoptosis and Synergizes with Cisplatin or Paclitaxel in Human Ovarian Cancer Cells

Figure 2

PL induced apoptosis in OVCAR3 ovarian cancer cells. (a) The results of cell apoptosis in PL-treated OVCAR3 cells. Cells were treated with the indicated concentration of PL for 48 hr, stained with Annexin V/PI, and examined by FCM. The proportions of Annexin V+/PI− and Annexin V+/PI+ cells indicated the early and late stages of apoptosis. (b) The quantified results of (a). (c) and (d) Representative Western blotting analysis of C-PARP in OVCAR3 cells treated with the indicated PL. β-actin was used as loading control. ^∗𝑃<0.05 and ^∗∗𝑃<0.01 versus corresponding control.

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