Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells

Figure 1

Nox4 nuclear localization and interaction in AFSC. (a) Representative images showing superimposing between DAPI (blue), Nox4 (green), and coilin (red) or sc-35 (red). Colocalization graph reporting Pearson’s and overlap coefficients. Scale bar: 10 μm. (b) Total lysates (TL) were immunoprecipitated with sc-35 antibody and then revealed with anti-sc-35 and anti-Nox4 (right) or were immunoprecipitated with anti-Nox4 and then revealed with anti-Nox4 and anti-sc-35 (left). Signals of preclearing sample (pcl) are shown in the middle line. (c) First line: representative images showing staining with nuclear ROS probe (nuclear peroxy emerald 1) of AFSC treated or not treated with shRNA. Second line: Nox4 signal (green) in the same samples. Scale bar: 10 μm. (d) Western blot revealed anti-Nox4 of AFSC treated with empty vector (ev) or siRNA TI311640, the best silencing vector among the 4 reported in Materials and Methods section. All presented data are representative of three independent experiments.

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