Nuclear Nox4 Role in Stemness Power of Human Amniotic Fluid Stem Cells

Figure 2

Nox4 interaction with transcription factors in nuclei of AFSC. (a) Representative images showing superimposing between DAPI (blue), Nox4 (green), and FoxO1 (red) or Oct4 (red). (b) Representative images showing superimposing between DAPI (blue), Oct4 (green), and coilin (red) or sc-35 (red). Scale bar: 10 μm. (c) Western blot analysis of nuclear lysate (NL) and immunoprecipitation experiment of NL with Nox4 antibody then revealed with anti-Oct4 and anti-sc-35. Signals of preclearing sample (pcl) are shown in the middle line. Presented data are representative of three independent experiments.

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