Regulation of System by Pharmacological Manipulation of Cellular Thiols

Figure 5

DEM and BSO cause a concentration dependent decrease in cellular glutathione levels. Cultures were exposed to varying concentrations of DEM or BSO for (a) 40 min, (b) 6 hrs, or (c) 24 hrs, after which cellular reduced glutathione was determined by MCB fluorescence. Bars show % MCB fluorescence normalized to control fluorescence (mean + SEM, = 8–16). indicates significant difference from control. < 0.05.

(a) 40 min

(b) 6 hour

(c) 24 hour