Cardiac and Vascular Synergic Protective Effect of Olea europea L. Leaves and Hibiscus sabdariffa L. Flower Extracts

Figure 3

Effect of OEE, HSE, and their mixture on intracellular ROS production. HUVECs were treated with OEE (a), HSE (b), and their mixture (c) (0.05–100 μg/mL) for 24 h; oxidative damage was then induced exposing the cells to 100 μM H₂O₂ for 30 min and intracellular ROS were determined using the peroxide-sensitive fluorescent probe DCFH-DA as described in Methods section. Data are expressed as percent of control cells treated with H₂O₂. Values represent means ± S.D. of four independent experiments. with respect to H₂O₂-treated cells.

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