Toll-Like Receptor 4 Promotes NO Synthesis by Upregulating GCHI Expression under Oxidative Stress Conditions in Sheep Monocytes/Macrophages

Figure 5

TLR4 enhanced GCHI activity in monocytes/macrophages and serum. The activities of NADPH oxidase, iNOS, and GCHI in monocytes/macrophages were examined after LPS stimulation ((a), (b), and (c), resp.). (d) Levels of TLR4 in the serum after LPS stimulation were measured by ELISA. (e) The effects of LPS stimulation on the expression of immune factors (TNF-α, IFN-γ, IL-6, IL-8, IL-12, and IL-10) in serum. (f) GCHI expression in serum was detected by ELISA. Tg: transgenic sheep, WT: wild-type. Data are expressed as mean ± SE; in Tg versus WT groups.

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