The Synergistic Effects of Heat Shock Protein 70 and Ginsenoside Rg1 against Tert-Butyl Hydroperoxide Damage Model In Vitro

Figure 1

Effects of pEGFP-HSP70, t-BHP, and G-Rg1 on SH-SY5Y cell viability. (a) The expression of HSP70 secreted by SH-SY5Y was captured by light microscope in C (media control), pEGFP (transfected with pEGFP in media), and pEGFP-HSP70 (transfected with pEGFP-HSP70 in media) groups. (b) Cells were subjected to the MTT assay to detect whether our recombination plasmid pEGFP-C2-HSP70 affected cell viability in pEGFP-HSP70 compared to C. Statistical comparisons were carried out with Student’s -test. Values were presented as mean ± SEM. . (c) HSP70 protein was detected by Western Blot analysis. Statistical comparisons were carried out with Student’s -test. Values were presented as mean ± SEM. ; experimental group versus medium control. . (d) Compared to media control group, cells after being transfected were used to the MTT assay for viability analysis, respectively. Statistical comparisons were carried out with Student’s -test. Values were presented as mean ± SEM. . The two groups which were transfected with pEGFP and pEGFP-HSP70 versus medium control. (e) SH-SY5Y cells were treated with t-BHP at various concentrations (0, 125, 250, 500, 750, 1000, and 1500 μmol/L) for 1 hour. (f) G-Rg1 at various concentrations for 24 hours. Cells were subjected to the MTT assay for viability analysis, respectively. Statistical comparisons were carried out with one ANOVA. Values were presented as mean ± SEM. versus medium group. .

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