The Synergistic Effects of Heat Shock Protein 70 and Ginsenoside Rg1 against Tert-Butyl Hydroperoxide Damage Model In Vitro

Figure 2

Functional properties of HSP70 and G-Rg1 on t-BHP-induced SHSY5Y cell injury. (a) ROS production in SHSY-5Y was determined using BD FACS. (b) ROS production was shown by DCF fluorescence in C′ (media control), T′ (10 μmol/L t-BHP), G′ (10 μmol/L G-Rg1 and 10 μmol/L t-BHP), H′ (SHSY-5Y cultured in 10 μmol/L t-BHP after being transfected with pEGFP-HSP70), P′ (SHSY-5Y cultured in 10 μmol/L t-BHP after being transfected with pEGFP), and HG′ (SH-SY5Y cultured in 10 μmol/L G-Rg1 and 10 μmol/L t-BHP after being transfected with pEGFP-HSP70), respectively. Statistical comparisons were carried out with Student’s -test. Values were presented as mean ± SEM. . T′, G′, H′, P′, and HG′ versus C′; versus T′. (c) DNA fragment in SH-SY5Y cells was determined using fluorescence microscope. (d) DNA fragment was described by tail lengths which were assessed by CAMP software in C′, T′, G′, H′, P′, and HG′, respectively. Statistical comparisons were carried out with Student’s -test. Values were presented as mean ± SEM. . T′, G′, H′, P′, and HG′ versus C′; versus T′. (e) SH-SY5Y cells were then subjected to apoptosis analysis. Apoptosis of neurons was determined by Western blot analysis of cleaved Caspase-3 levels. Statistical comparisons were carried out with Student’s -test. Values were presented as mean ± SEM. versus C′; versus T′. .

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