The Synergistic Effects of Heat Shock Protein 70 and Ginsenoside Rg1 against Tert-Butyl Hydroperoxide Damage Model In Vitro

Figure 3

G-Rg1 stimulated NSCs proliferation. (a) Immunofluorescence analysis of hippocampal NSCs cultures treated with media in 3rd day, stained with antibodies against the NSC-specific Nestin (green), and nuclei were counterstained with Hoechst 33342 (blue). (b) The nestin positive cells were counted by Image J software; statistical comparisons were carried out with Independent -test. Values were represented as mean ± SEM. Nestin positive cells versus Hoechst-stained cells. . (c) The MTT assay indirectly showed the OD value of NSCs proliferation for 7 days after being treated with different concentration of G-Rg1. Statistical comparisons of repeated multiple comparisons were carried out with Univariate Analysis; Statistical comparisons of multiple comparisons in the group of each time point were, respectively, carried out with Student’s -test. Values were represented as mean ± SEM. . (d) The MTT assay was described by Profile Plots.

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