The Synergistic Effects of Heat Shock Protein 70 and Ginsenoside Rg1 against Tert-Butyl Hydroperoxide Damage Model In Vitro

Figure 4

Efficiency of transfection of NSCs used pEGFP-C2-HSP70 vector. (a) Efficiency of transfection was confirmed by visualizing EGFP expression after transfection in 1, 3, and 7 days. Flow cytometry was performed to detect the fluorescence intensity of EGFP in Day 1, Day 3, and Day 7. (b) Fluorescence intensity of EGFP assessed by flow cytometry was described by Profile Plots. (c) Western Blot was performed to detect HSP70 protein after transfection in Day 1, Day 3, and Day 7. Statistical comparisons were carried out with Independent -test. Values were presented as mean ± SEM. versus control in Day 3; versus control in Day 7.

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