The Synergistic Effects of Heat Shock Protein 70 and Ginsenoside Rg1 against Tert-Butyl Hydroperoxide Damage Model In Vitro

Figure 5

Properties of NSCs after being transfected with pEGFP-HSP70 or/and treated with G-Rg1. (a) Representative images were shown at 100x magnification to show some morphological changes of NSCs after being transfected with pEGFP-HSP70 or/and treated with G-Rg1 in Day 1, Day 3, and Day 7, such as neurospheres and branches. (b) The MTT assay showing the OD value of NSCs after being transfected with p EGFP-HSP70 in Day 1, Day 3, and Day 7. Cell viability was not significantly different between control and pEGFP-HSP70 groups at . Statistical comparisons were carried out with Independent -test. Values were represented as mean ± SEM. . (c) The MTT assay showed the viability value of NSCs in Day 1, Day 3, and Day 7 after being transfected with p EGFP-HSP70 or/and treated with G-Rg1. versus media control in Day 3. versus G-Rg1 in Day 3. versus media control in Day 7. versus control in Day 7. Statistical comparisons of repeated multiple comparisons were carried out with Student’s -test. Mean ± SEM. . (d) Immunostaining with antibodies against the cholinergic neuron-specific ChAT (red), nuclei was counterstained with Hoechst 33342 (blue) and EGFP fluorescence was green. (e) And (f) Western Blot was, respectively, performed to detect HSP70 and ChAT expression in control, G-Rg1, pEGFP-HSP70 or/and treated with G-Rg1 groups in Day 1, Day 3, and Day 7. Statistical comparisons were carried out with Student’s -test. Values were presented as mean ± SEM. versus C. .

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