The Synergistic Effects of Heat Shock Protein 70 and Ginsenoside Rg1 against Tert-Butyl Hydroperoxide Damage Model In Vitro

Figure 6

Neuronal cell count and cell apoptosis. (a) Representative images of immunofluorescence were shown at 200x magnification to demonstrate the MAP-2 positive neurons in cotreatment on t-BHP-induced injury. TRITC (red)-labeled MAP-2 immunoreactions were shown in the cytoplasm; Nuclei was stained with Hoechst 33342 (blue) which as a specific marker of apoptosis was applied to determine chromatin condensation. Cells with intensely stained, condensed, or fragmented nuclei were scored as apoptotic. The arrowheads indicate remarkable apoptotic cells. C: media control; T: 10 μmol/L t-BHP; NHG: cocultured with NSCs which were transfected with pEGFP-HSP70 and G-Rg1 which dissolved in 10 μmol/L t-BHP. (b) The merged images of (a) pictures were magnified which were convenient for observation. (c), (d) Neuronal cell count and the percentage of apoptotic neurons were scored in Day 1, Day 3, and Day 7 for ascertaining the time points of study. Statistical comparisons of repeated multiple comparisons were carried out with Student’s -test; statistical comparisons of multiple comparisons in the group of each time point were, respectively, carried out with Student’s -test. Mean ± SEM. versus C in Day 1, versus C in Day 3, versus C in Day 7, versus T in Day 1, versus T in Day 3, and versus T in Day 7. .

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