TrkB-Mediated Neuroprotective and Antihypoxic Properties of Brain-Derived Neurotrophic Factor

Figure 2

Spontaneous calcium activity in dissociated hippocampal cultures in the posthypoxic period. (a) Example of a frame from the calcium-dependent fluorescent dye image series; green, cells marked with Oregon Green; yellow, propidium iodide-positive cells. (b) Spontaneous Ca²⁺ oscillation recordings in marked neurons. (B1-B2) Ca²⁺ oscillations in cells stained with Oregon Green; (B3) Ca²⁺ oscillations in cells stained with propidium iodide. ((c1)–(c3)) The main parameters of calcium activity in dissociated hippocampal cultures after 7 days of hypoxia. (c1) proportion of cells exhibiting calcium activity; (c2) duration of Ca²⁺ oscillations; (c3) number of Ca²⁺ oscillations per min (ANOVA , ).

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