Doses of Quercetin in the Range of Serum Concentrations Exert Delipidating Effects in 3T3-L1 Preadipocytes by Acting on Different Stages of Adipogenesis, but Not in Mature Adipocytes

Table 1

Primers for PCR amplification of each studied gene.

	Sense primer	Antisense primer	Probe
SYBR Green; RT-PCR:
LPL	5′-CAG CTG GGC CTAACT TTG AG-3′	5′-CCT CTC TGC AAT CAC ACG AA-3′
PPARγ	5′-ATT CTG GCC CAC CAA CTT CGG-3′	5′-TGG AAG CCT GAT GCT TTA TCC CCA-3′
SREBP1c	5′-AAA TCT TGC TGC CAT TCG-3′	5′-TTG ATC CCG GAA GCT CTG TG-3′
CEBPα	5′-TTC CTC CGG CTA AGA CTT AGG C-3′	5′-CAG GGG TGT GTG TAT GAA CTG G-3′
ATGL	5′-CAC TTT AGC TCC AAG GAT GA-3′	5′-TGG TTC AGT AGG CCA TTC CT-3′
HSL	5′-GGT GAC ACT CGC AGA AGA CAA TA-3′	5′-GCC GCC GTG CTG TCT CT-3′
FASN	5′-AGC CCC TCA AGT GCA CAG TG-3′	5′-TGC CAA TGT GTT TTC CCT GA-3′
ACC	5′-GGA CCA CTG CAT GGA ATG TTA A-3′	5′-TGA GTG ACT GCC GAA ACA TCT C-3′
PGC1α	5′-CCA AAG CTG AAG CCC TCT TGC-3′	5′-GTT TAG TCT TCC TTT CCT CGT GTC C-3′
Leptin	5′-TGG ACC AGA CTC TGG CAG TC-3′	5′-AGG ACA CCA TCC AGG CTC TC-3′
Adiponectin	5′-TG TAG GAT TGT CAG TGG ATC TG-3′	5′-GCT CTT CAG TTG TAG TAA CGT CAT C-3′
Apelin	5′-ATT TAA GGA CAC GCT GAT CAA AGG-3′	5′-AGT CCC GAA AGT ATT CAA AAG CAG-3′
β-actin	5′-ACG AGG CCC AGA GCA AGA G-3′	5′-GGT GTG GTG CCA GAT CTT CTC-3′
TaqMan; RT-PCR:
CEBPβ	5′-GAG CGA CGA GTA CAA GAT GCG-3′	5′-GCT GCT CCA CCT TCT TCT GC-3′	5′-FAM-TCG TTC TCC GCC GTC AGC TCC AGC-TAMRA-3′
SIRT-1	5′-GAC GAC GAG GGC GAG GAG-3′	5′-ACA GGA GGT TGT CTC GGT AGC-3′	5′-FAM-CTG CCG CCG CCG CTG CCG-TAMRA-3′
Visfatin	5′-CCG GCC CGA GAT GAA TGC-3′	5′-GGA ATA AAC TTT GCT TGT GTT GGG-3′	5′-FAM-AGC CGA GTT CAA CAT CCT GCT GGC-TAMRA-3′
-actin	5′-TCT ATG AGG GCT ACG CTC TCC-3′	5′-CAC GCT CGG TCA GGA TCT TC-3′	5′-FAM-CCT GCG TCT GGA CCT GGC TGG C-TAMRA-3′

LPL = lipoprotein lipase, PPAR = peroxisome proliferator-activated receptor, SREBP = sterol regulatory element-binding protein, C/EBP and C/EBP = CCAAT-enhancer-binding proteins alpha and beta, ATGL = adipose triglyceride lipase, HSL = hormone sensitive lipase, FASN = fatty acid synthase, ACC = acetyl-CoA carboxylase, PGC-1 α = peroxisome proliferator-activated receptor gamma coactivator, and SIRT1 = deacetylase sirtuin 1.