miR-103 Regulates Oxidative Stress by Targeting the BCL2/Adenovirus E1B 19 kDa Interacting Protein 3 in HUVECs

Figure 3

Validation of BNIP3 as a miR-103 target gene. (a) The 3′-UTR of BNIP3 and mutant 3′-UTR sequences that abolished binding. (b) Luciferase activity was assessed in HUVECs transfected with BNIP3 3′-UTR-WT or BNIP3 3′-UTR-MUT and the mimic control or miR-103. (c) Luciferase activity was assessed in HUVECs transfected with BNIP3 3′-UTR-WT or BNIP3 3′-UTR-MUT and the inhibitor control or miR-103 inhibitor. (d) BNIP3 mRNA levels analyzed by qRT-PCR. (e) Western blot analysis of the endogenous expression of BNIP3 upon forced expression of miR-103. (f) The protein expression of BNIP3 in HUVECs transfected with the miR-103 inhibitor or inhibitor control was determined by western blotting. ; .

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