DNA Damage and Inhibition of Akt Pathway in MCF-7 Cells and Ehrlich Tumor in Mice Treated with 1,4-Naphthoquinones in Combination with Ascorbate

Figure 2

ROS were measured in MCF-7 cells treated for 2 h with juglone, Q7 or Q9 at 10 μM with or without ascorbate 1 mM (a). Integrity of PARP protein in MCF-7 cells treated with juglone (Jug), Q7 or Q9 at 20 μM with or without ascorbate (Asc) 1 mM for 24 h. Sanguinarine (Sang) 5 μM was used as a positive control of apoptosis (b). Colony-forming units of MCF-7 cells treated with juglone, Q7 or Q9 at 10 μM with/without ascorbate 1 mM for 2 h (c). Phosphorylated Akt (pAkt) was assessed by immunoelectrophoresis in MCF-7 cells treated for 24 h (d). Data were obtained from three independent experiments. () and () denote statistical differences at and compared to nontreated control cells or between indicated treatments, respectively.

(a)

(b)

(c)

(d)