Intracellular Oxidant Activity, Antioxidant Enzyme Defense System, and Cell Senescence in Fibroblasts with Trisomy 21

Figure 10

Effect of cell passages on cytochrome c and caspase 9 expression in fibroblasts with trisomy 21 (T21F) () and controls (CF) (). (a) Representative Western blot of cytochrome c and caspase 9 at low and high passages. (b and c) Protein expression was calculated from optical densities of the bands, measured by an imaging technique and normalized by β-actin. Results were normalized to the band intensities measured in untreated fibroblasts at low passages and expressed as mean ± SEM. Statistical differences were analyzed by Student’s -test: .

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