Intracellular Oxidant Activity, Antioxidant Enzyme Defense System, and Cell Senescence in Fibroblasts with Trisomy 21

Figure 6

Effect of cell passages on SOD1 and SOD2 expression in fibroblasts with trisomy 21 (T21F) () and controls (CF) (). SOD1 and SOD2 expression were analyzed by Western blot (a) and the bands were quantified by an imaging technique and normalized by β-actin (b and c). Results are normalized to the band intensities measured in untreated fibroblasts at low passages and expressed as mean ± SEM. Statistical differences were analyzed by Student’s -test: T21F versus CF (; ); high versus low passages ().

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