Oxidative Stress and Adipocyte Biology: Focus on the Role of AGEs

Figure 3

Adipocyte cell line expresses CD36 receptor. SW872 adipocytes were cultured on 12 mm diameter poly(L-lysine) coverslips until 80% confluency. Cells were fixed in diluted paraformaldehyde solution (4% v/v in PBS) at room temperature for 20 min and stained successively with a primary human CD36 antibody (1 : 50 in 1% PBS/BSA) for one hr, followed by incubation with an Alexa Fluor 488 goat anti-rabbit IgG antibody (1 : 1,000) for 1 hr and with DAPI solution (1 : 1,000) for 10 min (for staining of the nucleus). Cells were washed 3x in 1% PBS/BSA between each step of different incubation periods. Cells were imaged using a Nikon eclipse microscope and NIS-Element software (Nikon Corporation, Tokyo, Japan). (vs. HSA) by Student’s unpaired test ().