A Novel Antioxidant Multitarget Iron Chelator M30 Protects Hepatocytes against Ethanol-Induced Injury

Figure 3

M30 improved cellular oxidative stress. (a) Production of ROS staining in the cells after various treatments (magnification 200x, bar = 100 μm). (b) Quantification of ROS staining. (c) Expressional changes of antioxidants CAT and GPx1 after various treatments. (d) Change of GSH/GSSG ratio treatments. (e) Changes of transcriptional activity of HIF-1α in each group. Data from each group () were expressed as means ± SEM. Statistical comparison between groups was done using the Kruskal-Wallis test followed by Dunn’s post hoc test to detect differences in all groups. “” means significantly different from control group (). “” means significantly different from control group (). “” means significantly different from control group (). “” means significantly different from ethanol exposure group (). “” means significantly different from ethanol exposure group (). Letters “a” labelled above the values of control, M30, and ethanol + M30 groups mean there is no significant change among these groups (). Letter “b” means the value of ethanol-treated group is statistically different from other three values (). EtOH, ethanol, M30, vehicle M30 treatment, E + M30, ethanol + M30 cotreatment.

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