Potential Therapeutic Role of L-Carnitine in Skeletal Muscle Oxidative Stress and Atrophy Conditions

Figure 4

CARN action during myoblast differentiation: enhancing myotubes formation. (a) CARN enhances myotube formation rising MyHC and myogenin protein levels. (b) MyHC immunofluorescence assay revealed that CARN is able to enhance myotubes formation. (c) Phase contrast images at the end of differentiation show the important morphological changes and dimensional increment in neoformed myotubes after treatment with CARN. Representative immunoblots of analyzed proteins are shown. Scale bar 200 μm. Significance: Anova test and DM versus CARN at 24 h, 48 h, and 72 h.

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