Research Article
Polymethoxyflavone Apigenin-Trimethylether Suppresses LPS-Induced Inflammatory Response in Nontransformed Porcine Intestinal Cell Line IPEC-J2
Table 1
Sequence of primer sets used for quantitative real-time.
| Gene symbol | Accession number | Primer sequences | Product size (bp) | Efficiency | | Reference |
| IL-8 | NM_213867 | F 5′-AGAGGTCTGCCTGGACCCCA-3′ | 126 | 1.972 | 0.999 |
[28] | R 5′-GGGAGCCACGGAGAATGGGT-3′ |
| IL-6 | NM_214399 | F 5′-TTCACCTCTCCGGACAAAAC-3′ | 122 | 1.970 | 0.995 |
[29] | R 5′-TCTGCCAGTACCTCCTTGCT-3′ |
| TNF- | NM_214022 | F 5′-TTCCAGCTGGCCCCTTGAGC-3′ | 146 | 1.873 | 0.982 |
[30] | R 5′-GAGGGCATTGGCATACCCAC-3′ |
| COX-2 | NM_214321 | F 5′-AGAAGCGAGGACCAGCTTTC-3′ | 215 | 1.905 | 0.981 | NCBI/Primer-Blast | R 5′-AAAGCGGAGGTGTTCAGGAG-3′ |
| Hsp70 | NM_001123127 | F 5′-GCCCTGAATCCGCAGAATA-3′ | 152 | 2.0 | 0.993 |
[31] | R 5′-TCCCCACGGTAGGAAACG-3′ |
| CycA | NM_214353 | F 5′-GCGTCTCCTTCGAGCTGTT-3′ | 160 | 1.907 | 0.998 |
[30] | R 5′-CCATTATGGCGTGTGAAGTC-3′ |
| HPRT | NM_001032376 | F 5′-GGACTTGAATCATGTTTGTG-3′ | 91 | 1.963 | 0.997 |
[32] | R 5′-CAGATGTTTCCAAACTCAAC-3′ |
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