Hydrogen Sulfide Donor GYY4137 Protects against Myocardial Fibrosis

Figure 8

Effect of GYY4137 on Ang II-induced oxidative stress in cardiac fibroblasts. Neonatal rat cardiac fibroblasts were pretreated with different concentrations of GYY4137 for 4 h followed by Ang II (10⁻⁷ M) stimulation for an additional 24 h. (a–e) Levels of ROS in cardiomyocytes were measured by DCFH-DA (10 μM) fluorescence staining (scale bar: 100 μm). (a) Cells treated with culture medium served as vehicle control (con.); (b) Ang II stimulation alone; (c-d) cells pretreated GYY4137 (12.5 μM, 25 μM, and 50 μM), respectively, for 4 h followed by Ang II (10⁻⁷ M) stimulation for an additional 24 h. (f) Fluorescence was estimated directly by flow cytometry. Plots represent mean ± SEM from 4 independent experiments. Cells treated with culture medium served as vehicle control (con.). Statistical significance: , compared with con. group; , compared with Ang II stimulation alone.

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