Melatonin Attenuates Oxidative Damage Induced by Acrylamide In Vitro and In Vivo

Figure 1

Effects of MT on cytotoxicity induced by ACR in PC12 cells. Cell viabilities were determined as relative percent of viable cells by MTT method. PC12 cells were incubated with different concentrations of ACR (1.25, 2.5, and 5 mM) for 1, 3, 6, 12, and 24 h (a) or MT (0, 25, 50, 100, 200, 400, and 800 μM) for 24 h (b). Similarly, cell viabilities were analyzed in the vehicle control group, ACR alone treatment group, ACR + MT (50 μM) cotreatment group, and ACR + MT + luzindole (0.2 μM) cotreatment group for 24 h (c). The results are expressed as the mean ± SD (). , versus the vehicle control group. ,   versus the ACR treatment group, ,   versus the ACR + MT cotreatment group.

(a)

(b)

(c)