Sca-1+ Cells from Fetal Heart with High Aldehyde Dehydrogenase Activity Exhibit Enhanced Gene Expression for Self-Renewal, Proliferation, and Survival

Figure 2

Identification of subpopulation in cultured fetal Sca-1+ cells using activity based flow cytometric sorting. There is a distinct and consistent Sca-1+ cell population (10–15%) in fetal heart. Dot plots have been shown for three human fetal Sca-1+ cell samples. Left panel represents negative control for each sample. Right panel is the test sample in the absence of the inhibitor, DEAB. ALDH activity is measured in the FITC channel, represented along -axis; forward scatter (FSC) is on the -axis.

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