Cardiac H₂S Generation Is Reduced in Ageing Diabetic Mice

Figure 7

Exogenous H₂S attenuates high glucose-induced myocardial injury. (a) Exogenous NaHS caused transient increase of H₂S level in cell culture media within 6 hours. versus HG group; versus HG group ( in each group). (b) H₂S levels in neonatal rat cardiac ventricular myocytes (NRCMs) at the end of the treatment for 72 h. (c) NRCMs viability measured by CCK-8 assay at the end of the treatment for 72 h. (d) Representative images of cardiomyocyte apoptosis detected by a laser confocal microscope at the end of the treatment for 72 h. (e) Quantitative analysis for cardiomyocyte apoptosis determined by flow cytometry. (f) Representative images of ROS levels in NRCMs detected by a laser confocal microscope at the end of the treatment for 72 h. (g) Quantitative analysis for ROS levels in NRCMs. NG group, normal glucose (5.5 mmol/L); HG group, high glucose (33 mmol/L). Results are means ± SEM. A of <0.05 was considered significant.

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