GC-Rich Extracellular DNA Induces Oxidative Stress, Double-Strand DNA Breaks, and DNA Damage Response in Human Adipose-Derived Mesenchymal Stem Cells

Figure 7

Mitochondrial biogenesis increased with adipogenic differentiation of haMSCs in the presence of GC-DNA. (a) HaMSCs were grown to subconfluency (~80%) and then DNA samples were added. Cells were cultivated in the presence of DNA samples (50 ng/mL) at 37°C for 7 days in AmnioMax Basal Medium with AmnioMax Supplement C100 (Gibco). (A) Cells were stained with MitoTracker TMRM and were photographed at the same exposure (20x). (B) FACS analysis showing the distribution of fluorescence intensities of the cells stained with TMRM. (b) Cells were cultivated in the presence of DNA samples (50 ng/mL) at 37°C for 14 days; in a week the culture medium with DNA samples was refreshed. Cells were stained with MitoTracker TMRM and Hoechst 33342 (100×).

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