The Neuroprotective Effects of Ratanasampil on Oxidative Stress-Mediated Neuronal Damage in Human Neuronal SH-SY5Y Cells

Figure 3

The effects of RNSP on H₂O₂-induced DNA damage in SH-SY5Y cells. (a) Fluorescent images of MitoSOX Red signals in the H₂O₂-incubated cells (100 μM, 1 h) with or without RNSP (60 μg/mL, 2 h). Scale bar = 10 μm. (b) REST expression, as analyzed by real-time quantitative RT-PCR, in the H₂O₂-exposed cells (100 μM, 1 h, 2 h), with or without RNSP (60 μg/mL, 2 h). Each column and bar represent the mean ± SEM ( each). Asterisks indicate a statistically significant difference from the value in the untreated cells (, ). Pound signs indicate a statistically significant difference from the value in the H₂O₂-incubated cells without RNSP (^#, ^##). (c) Immunofluorescent CLMS images of 8-oxo-dG (green) with Hoechst-stained nuclei (blue) in the H₂O₂-exposed cells (100 μM 4 h), with or without RNSP (60 μg/mL, 2 h). Scale bar = 10 μm. (d) The quantitative analyses of 8-oxo-dG immunofluorescence signal intensity in (c). Each column and bar represent the mean ± SEM ( each). Asterisks indicate a statistically significant difference from the value in the untreated cells (). Pound signs indicate a statistically significant difference from the value in the H₂O₂-exposed cells without RNSP (^###).

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