Research Article
Quercetin Affects Erythropoiesis and Heart Mitochondrial Function in Mice
Figure 4
Quercetin treatment affects complexes OXPHOS properties in mice isolated mitochondria. Heart mitochondria isolated from quercetin-treated mice were digitonin solubilized and fractionated by BN-PAGE and then followed by Complex I (a), Complex II (b), and Complex IV (c) in-gel activity assays. (a) IGA Complex I densitometry analysis for supercomplex and monomer fraction. (b) IGA Complex IV densitometry analysis for supercomplex and monomer fraction. Images show three independent experiments. Each bar represents the mean ± SD, analyzed by two-sample -test (). Abbreviations used are as follows: blue native polyacrylamide gel electrophoresis (BN-PAGE), Complex I (CI), Complex IV (CIV), in-gel activity assay (IGA), and supercomplexes (SC). SC1:CI:CIII2:CIV1−4; SC2:CIII2:CIV1−4.
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