Schisandrae Fructus Supplementation Ameliorates Sciatic Neurectomy-Induced Muscle Atrophy in Mice

Figure 7

Representative gastrocnemius muscle caspase-3, PARP, nitrotyrosine, 4-HNE, iNOS, COX-2, TNF-α, and myostatin immunoreactivities in samples taken from intact mice or mice with NTX-induced muscle atrophy. After deparaffinization of prepared gastrocnemius muscle histological paraffin sections, citrate buffer antigen (epitope) retrieval pretreatment was conducted. Thereafter, the sections were immunostained using ABC methods for caspase-3, PARP, nitrotyrosine, 4-HNE, iNOS, COX-2, TNF-α, and myostatin ((a) intact vehicle control mice; (b) NTX control mice; (c) 50 mg/kg oxymetholone-treated mice; (d) 500 mg/kg EESF orally treated mice; (e) 250 mg/kg EESF mg/kg orally treated mice; (f) 125 mg/kg EESF orally treated mice; Scale bars = 40 m).

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