Upregulation of Multidrug Resistance-Associated Protein 1 by Allyl Isothiocyanate in Human Bronchial Epithelial Cell: Involvement of c-Jun N-Terminal Kinase Signaling Pathway

Figure 3

Effect of JNK cell signaling pathway inhibitor on MRP1 function in 16HBE14o- cells. (a) Cells were pretreated with or without the addition of SP600125 (20 μM) for 60 min and then incubated with AITC (40 μM) for 24 h and the fluorescence of the CF retained within the cells was evaluated by flow cytometry. (b) Intracellular CF (MFI) retention as a measure of MRP1 function in 16HBE14o- cells. Significant difference from control, . Significant difference from CFDA, . Significant difference from CFDA, . Significant difference from AITC alone, . Significant difference from AITC alone, .

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(b)