Allopurinol Protects against Ischemia/Reperfusion-Induced Injury in Rat Urinary Bladders

Figure 6

Detection of in situ superoxide in bladder tissue by DHE staining. I/R + S group showed significantly increased signal intensities compared with Sham + S group. Compared with I/R + S group, the DHE fluorescence intensity in the bladder of I/R + Allo group was significantly decreased. Images were acquired at identical settings and are representative of similar results obtained from three independent experiments. (a) Sham + S; (b) I/R + S; (c) I/R + Allo. The scale bar indicates 50 mm (bottom). (d) Intensity values are expressed as arbitrary fluorescence units. indicates that I/R + S group is significantly different from sham-operated groups (). † indicates that I/R + Allo group is significantly different from I/R + S groups (). Bars indicate mean ± SEM.

(a)

(b)

(c)

(d)