Anticancer Properties of Phyllanthus emblica (Indian Gooseberry)

Figure 1

Cytotoxic effects of Phyllanthus emblica  (Indian Gooseberry)  extract against triple-negative breast cancer cells. (a) MCF10A cells represent untransformed mammary epithelial cells. All other cell lines represent triple-negative breast cancer cell lines. Growth media for all cell lines were used according to ATCC recommendations. 10⁴ cells were seeded in 24-well plates. 24 hours after plating, Indian Gooseberry extract (Saberry, Sabinsa Corporation) dissolved in PBS was added daily to fresh media at the indicated concentrations or PBS to control cells. Cell viability was measured using trypan blue exclusion with a hemocytometer after a five-day exposure period. Experiments were carried out multiple times in triplicate. Error bars represent SEM. P values for growth inhibition of MDA-MB-435, MDA-MB-468, MDA-MB-231, and BT20 cells exposed to 100 µg/mL Phyllanthus emblica compared to control cells were all <0.05 (denoted by ). MCF10A cells exposed to the same concentration showed no significant change. (b) Visualization of MDA-MB-468 cells after five-day exposure to Phyllanthus emblica extract at 10x magnification.

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