Antioxidant, Antibacterial, and Cytotoxic Activities of the Ethanolic Origanum vulgare Extract and Its Major Constituents

Figure 5

The antioxidant effect of (a) carvacrol and thymol and (b) thymol and/or carvacrol. Equimolar concentration to carvacrol and thymol is present in the extract at the 2.93 μg/mL concentration, as well as in ethanolic extract against H₂O₂-induced cytotoxicity in A549 cells. All experiments were repeated three times in two replicates as outlined in Section 2. (A) denotes significance from the control group treated with H₂O₂ only, ; (B) denotes significance from the thymol pretreated group challenged with H₂O₂, ; denotes significance from the control group treated with H₂O₂ only, ; # denotes significance from the control group pretreated with thymol and carvacrol mixture and challenged with H₂O₂, .

(a)

(b)