Normolipidemic human blood LDL (0.25 mg/mL) in vitro oxidation in the presence of 5 M CuSO4 and CA of 3 types (DHPs, verapamil, and diltiazem) and vitamin E. Compiled according to Lupo et al. [129].
Compound
Methods
TBARS method (fluorimetry at 515 nm/533 nm, 4 hours preincubation of LDL with compounds and copper (II) ions; 320% TBARS increase in control during 4 h period)
Inhibition of conjugated diene formation (at 234 nm) expressed as prolongation of induction period (in %% of control). = 36.8 min.
Effective [IC] (in M): 1 M; 10 M; 50 M
Activity rank order (ARO = I for the highest activity; ARO = VII for the mindest activity)
Effective [IC] (in M): 1 M; 5 M; 10 M; 50 M
Activity rank order (ARO = I for the highest activity; ARO = VII for the mindest activity)