Fisetin Modulates Antioxidant Enzymes and Inflammatory Factors to Inhibit Aflatoxin-B1 Induced Hepatocellular Carcinoma in Rats

Figure 3

Effect of Fisetin on expression (a) and activity profile (b) of SOD1 in the liver from control (C), HCC, and Fisetin treated HCC rats. In (a), liver extract containing 60 µg protein in each lane was subjected to 10% SDS-PAGE followed by western transfer and detection of SOD1 bands against a polyclonal anti-SOD1. The photograph is representative of the three western blot repeats. Normalized densitometry values of SOD1/β-actin have been presented as mean ± SD. (b) shows 8% nondenaturing PAGE results of 40 µg protein loaded in each lane. After electrophoresis, gel was subjected to development of substrate specific SOD1 band. The gel photograph is representative of the four PAGE repeats. The relative densitometric values of SOD1 band have been presented as mean ± SD from the four PAGE repeats. (control versus HCC group) and , (HCC versus HCC + F group).

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