The Impact of Lipoprotein-Associated Oxidative Stress on Cell-Specific Microvesicle Release in Patients with Familial Hypercholesterolemia

Figure 1

Flow cytometric analysis of CD36-positive MVs derived from various cell types. MVs were identified by size (<1 μm) and by surface exposure of phosphatidylserine. Colabeling with cell-specific markers identified CD36 expressing MVs (colored red) from various cell types, including (a) CD36+ CD41+ (platelet-derived) MVs, (b) CD36+ CD235a+ (erythrocyte-derived) MVs, (c) CD36+ CD14+ (monocyte-derived) MVs, and (d-e) CD36+ CD31+/CD42b− (endothelial cell-derived) MVs identified by a two-color method. CD31 is expressed on both platelets and endothelial cells, whereas CD42b is restricted to platelets, allowing discrimination between PMVs and EMVs (colored blue). Right columns correspond to the respective isotype controls.