(Z)-5-(2,4-Dihydroxybenzylidene)thiazolidine-2,4-dione Prevents UVB-Induced Melanogenesis and Wrinkle Formation through Suppressing Oxidative Stress in HRM-2 Hairless Mice

Figure 1

MHY498 ameliorates UVB-induced skin pigmentation. MHY498 was pretreated for 3 days. From day 4, 2 h after MHY498 application to the dorsal skin of the hairless mice, UVB was exposed to the mice as shown in Table 1 (/group). After 4 weeks, pictures of the dorsal skin were taken (a). Darkness of the skin sites was measured on day 28 of the treatment using a CR-10 spectrophotometer (Konica Minolta Sensing, Inc., Sakai, Osaka, Japan). Higher values represent whiter colors (/group) (b). After MHY498 application followed by UVB exposure, skin samples were excised and Fontana-Masson staining was performed as described in Materials and Methods (c–f). Microscopic analysis of dorsal skin sections from (c) control mice without UVB exposure, (d) mice with UVB exposure, and (e) MHY498 (0.5 μM) or (f) MHY498 (5 μM) treated mice. Arrows represent where melanogenesis was induced by UVB. Data are represented as mean ± SEM. and compared to a control group without UVB exposure compared to control group with UVB exposure.

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