(Z)-5-(2,4-Dihydroxybenzylidene)thiazolidine-2,4-dione Prevents UVB-Induced Melanogenesis and Wrinkle Formation through Suppressing Oxidative Stress in HRM-2 Hairless Mice

Figure 3

MHY498 reduces CREB phosphorylation and MITF translocation into nucleus. MHY498 was pretreated for 3 days. From day 4, 2 h after MHY498 application to the dorsal skin of the hairless mice, UVB was exposed to the mice every other day for 1 h. After 4 weeks, skin samples were excised and homogenated. Western blotting was performed using skin tissues for detecting (a) p-CREB, CREB, MITF, and TFIIB in nucleus fraction and (d) tyrosinase and β-actin in cytosol fraction (/group). The protein levels of (b) p-CREB, (c) MITF, and (e) tyrosinase were semiquantified using Image J software. p-CREB and MITF protein levels were normalized by TFIIB (/group). Tyrosinase protein levels were normalized by β-actin. A representative blot is shown from four experiments that yielded similar results. Data are represented as mean ± SEM. and compared to control group without UVB exposure.

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