Biofunctional Activities of Equisetum ramosissimum Extract: Protective Effects against Oxidation, Melanoma, and Melanogenesis
Table 1
Antioxidant activities of E. ramosissimum extracts, including reducing power, DPPH free radical scavenging activity, and ferrous ion chelating power.
Extracts (μg/mL)
Reducing power (OD700)
DPPH scavenging capacity (%)
Chelating activity (%)
50
100
200
50
100
200
50
100
200
EA
0.60 ± 0.06
0.79 ± 0.06
0.87 ± 0.23
<10.0
15.23 ± 2.76
43.41 ± 7.68
20.47 ± 3.57
34.69 ± 5.03
44.56 ± 1.32
DM
0.59 ± 0.03
0.65 ± 0.07
0.68 ± 0.04
<10.0
<10.0
<10.0
15.57 ± 1.39
17.49 ± 0.13
20.19 ± 1.72
Hex
0.56 ± 0.11
0.65 ± 0.10
0.68 ± 0.03
<10.0
<10.0
<10.0
17.98 ± 4.50
20.71 ± 0.33
22.53 ± 6.07
MeOH
0.58 ± 0.01
0.67 ± 0.13
0.73 ± 0.02
<10.0
10.69 ± 1.66
15.60 ± 1.28
10.63 ± 1.65
18.89 ± 1.19
20.43 ± 0.07
Water
0.62 ± 0.03
0.65 ± 0.10
0.70 ± 0.01
<10.0
<10.0
14.87 ± 0.73
10.77 ± 0.79
13.33 ± 4.55
13.52 ± 0.59
Vitamin C
—
—
—
—
85.55 ± 0.48
—
—
—
—
BHA
—
1.64 ± 0.29
—
—
—
—
—
—
—
EDTA
—
—
—
—
—
—
—
95.49 ± 0.05
—
All statistics are presented as average values ± SD; . Vitamin C (100 mM) was utilized as a positive control for DPPH assay; EDTA (100 mM) was used as a positive control for analysis of metal chelating ability; BHA (100 mM) was applied as a positive control for analysis of reducing power. Assays not performed in this study were indicated by dashes.