Maladaptive Modulations of NLRP3 Inflammasome and Cardioprotective Pathways Are Involved in Diet-Induced Exacerbation of Myocardial Ischemia/Reperfusion Injury in Mice

Figure 1

Cardiac metabolic adaptations to diet. Representative western blotting showing cardiac levels of α-MHC and β-MHC isoforms (a) and of markers of oxidative metabolism CPT-1 and SDH (b) assessed after 12 weeks of Control or HFHF diet in heart apex extracts. Histograms report densitometric analysis of 10–12 mice per group normalized for the corresponding tubulin content. versus Control. (c) Representative 20x/40x magnification photomicrographs of heart apex sections from Control or HFHF diet mice showing cardiac intramyocellular lipid accumulation by Oil Red O staining.

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