Maladaptive Modulations of NLRP3 Inflammasome and Cardioprotective Pathways Are Involved in Diet-Induced Exacerbation of Myocardial Ischemia/Reperfusion Injury in Mice

Figure 3

Localization and expression of GLUT-4 and IRS-2 activation in the mouse heart. Representative 40x magnification photomicrographs of heart apex sections and western blotting analysis on heart apex extracts from Control or HFHF diet mice, exposed or not to IR, showing GLUT-4 localization (a) and expression (b). Representative western blotting for cardiac levels of total IRS-2 and Ser270 phosphorylation performed on heart apex extracts from Control or HFHF diet mice, exposed or not to IR (c). Histogram reports densitometric analysis of the phosphorylated-to-total form ratio of 5-6 mice per group. versus Control; versus Sham.

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