The Relevance of Nrf2 Pathway and Autophagy in Pancreatic Cancer Cells upon Stimulation of Reactive Oxygen Species

Figure 4

Suppression of autophagic activity enhances Nrf2 expression and translocation of Nrf2 to the nucleus upon ROS stimulation in PANC-1 cells. (a) The expression of Beclin1 and LC3-II treated with or without 3-MA or CQ in the absence of ROS. (b) The expression of Nrf2, Beclin1, and LC3-II and also the level of p62 in pancreatic cancer cells under basal condition and upon ROS stimulation with or without 3-MA or CQ. (c) Immunofluorescence images of PANC-1 cells for Nrf2 under basal condition and upon ROS stimulation with or without 3-MA or CQ.

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