Exogenous Nitric Oxide Protects Human Embryonic Stem Cell-Derived Cardiomyocytes against Ischemia/Reperfusion Injury

Figure 2

Real-time PCR analysis of differentiated EBs (6 + 4 days of differentiation). OCT4 and NANOG pluripotency markers were downregulated (a), whereas mesodermal (BRACHYURY) and early and later cardiac markers (NKX2.5, GATA4, and ALCAM) were upregulated (b) as compared to the undifferentiated human HUES9 embryonic stem cell line control. Data are expressed as mean ± SEM; , and , differentiated EBs versus undifferentiated control; Student t-test, .

(a)

(b)