Targeting Pin1 Protects Mouse Cardiomyocytes from High-Dose Alcohol-Induced Apoptosis

Figure 5

Pin1 reduced the NO production and eNOS expression that were inhibited by alcohol in cardiomyocytes. (a) NO production in cardiomyocytes treated with alcohol (0, 50, 100, or 200 mM) for 24 h. (b) eNOS expression analyzed by western blotting in cells treated with alcohol at indicated concentrations. NO production (c) and eNOS expression (d) in Pin1-knockdown cardiomyocytes treated or untreated with alcohol (200 mM) for 24 h. NO production (c) and eNOS expression (d) in Pin1-overexpressed cardiomyocytes treated or untreated with alcohol (50 mM) for 24 h. and .

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