The C-ETS2-TFEB Axis Promotes Neuron Survival under Oxidative Stress by Regulating Lysosome Activity

Figure 5

TFEB protects cells from apoptosis under oxidative stress. (a, b, c) SHY5Y cells were infected with a TFEB-knockdown or a TFEB-overexpression adenovirus for 48 h and then treated with H₂O₂ (100 μM) for 12 h (for TUNEL staining) or 4 h (for western blotting). Apoptosis was detected by TUNEL staining and western blot analysis. The proportion of apoptotic cells was counted using ImageJ software, and 3–6 fields of each cover slide were analysed. The results are shown in (b). (d, e, f) Primary neurons were infected with a TFEB-knockdown adenovirus for 48 h and treated with H₂O₂ (150 μM) for 4 h. Apoptosis was detected by western blot analysis and flow cytometry. (f) shows the flow cytometry data. Values are the means ± SEM. compared with the control group.

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