The C-ETS2-TFEB Axis Promotes Neuron Survival under Oxidative Stress by Regulating Lysosome Activity

Figure 7

TFEB upregulation is controlled by C-ETS2 under oxidative stress. (a) TFEB promoter was truncated into three fragments and the luciferase activity of each fragment was assessed in 293T cells after treatment with H₂O₂ via a luciferase assay. Values are the means ± SEM. compared with the H₂O group. (b) C-ETS2 or C-ETS1 knockdown plasmids were transfected into 293T cells, and the transcription activity of TFEB was analysed by luciferase assay. Values are the means ± SEM. compared with the empty vector group. (c, d) C-ETS2 knockdown plasmids were transfected into 293T cells, and the TFEB mRNA and protein levels were measured by luciferase assay and western blot analysis. (e, f) TFEB expression was analysed in 293T cells overexpressing HA-C-ETS2 via western blot, RT-PCR, and luciferase assays. Values are the means ± SEM. compared with the empty vector group.

(a)

(b)

(c)

(d)

(e)

(f)